Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Measurement of Calcium by Ion Selective Electrode (CAT#: STEM-ACT-0101-WXH)

Introduction

Calcium, the most abundant mineral in the body, is found in some foods, added to others, present in some medicines (such as antacids), and available as a dietary supplement. Calcium makes up much of the structure of bones and teeth and allows normal bodily movement by keeping tissue rigid, strong, and flexible
The normal regulation of calcium in our blood stream is similar to the way a thermostat works. The body is set to have a normal amount of calcium (somewhere between 8.6 to 10.3 mg/dL). The parathyroid glands can be thought of as the “calcium thermostat” of the body.




Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode
Advertisement