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Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.
Advantages of using multiplex PCR include:
More information with less sample
Higher throughput
Cost effective — fewer dNTPs, enzymes, and other consumables
Time saving
Less input material required
More data from limited starting materials
Increased accuracy of data normalization
Fewer pipetting errors