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NGS analyses have revolutionized our understanding of biological processes. For critical studies, data obtained from next-generation sequencing systems should be confirmed. Sanger sequencing by CE is an ideal orthogonal technology for verification of NGS base calls.<br />Sanger sequencing is the gold standard of sequencing technology: it provides high accuracy, long-read capabilities, and flexibility to support a diverse range of applications. Sanger sequencing is mostly known for DNA sequencing applications, but also supports applications in RNA sequencing and epigenetic analysis.