Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!
This service presents the use of microreactors packed with immobilized trypsin particles for the rapid and efficient bottom-up analysis of proteins by on-line immobilized enzyme microreactor capillary electrophoresis mass spectrometry (IMER-CE-MS). Under the optimized conditions, using 10 mM NH4HCO3 pH 7.9 as the digestion buffer, a very low protein sample flow through the microreactor, a separation BGE of 50 mM HAc: 50 mM HFor, pH 2.3, and a temperature of digestion and separation of 37 °C, proteins were analyzed in less than 30 min (including all the steps).