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Observations on the peripheral vegetative nervous system by fluorescence microscope (CAT#: STEM-MIT-0176-LJX)

Introduction

The autonomic nervous system is the efferent nerve in the visceral nerve fibers, also known as the autonomic nerve. The autonomic nervous system is responsible for vital physiological functions such as heartbeat, respiration, digestion, blood pressure, metabolism and so on.
The autonomic nervous system is a control system that unconsciously regulates bodily functions such as heart rate, digestion, breathing rate, pupil response, urination, and sexual drive. The system controls "stress" and "emergency" responses.




Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Malignant lung tumor cells

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye
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