Predicted Light Scattering from Particles Observed in Human Age-Related Nuclear Cataracts Using Mie Scattering Theory (CAT#: STEM-ST-0072-YJL)

Introduction

Assessments of light-scattering by the ocular lens depend critically on the measurement geometry. For example, slit lamp images obtained by ophthalmologists detect light scattered mainly by small particles that preferentially scatter at high angles. Light scattered at low angles (forward scattering) is more difficult to measure in vivo, although indirect methods have been proposed, and some studies have reported in vitro measurements from isolated human lenses. Based on a theoretical analysis using the Rayleigh-Gans approximation of scattering from in vitro noncataractous lenses, it has been suggested that 1.4-μm diameter particles could account for the observed forward scattering. Spherical particles with diameters of 1 to 4 μm have been reported in transparent and cataractous human lens nuclei. Electron micrographs reveal that these particles are typically covered with a lipid-rich coat of concentric bilayers, hence the name multilamellar bodies (MLBs).

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Principle Applications Procedure Materials Notes Related Products

Principle

Mie scattering is defined as the type of scattering in which the diameter of the particle is the same or more than the wavelength of the radiation. Mie scattering gives a generalized solution for a system where a scattering of light takes place by a homogenous spherical medium. And this medium should have a refractive index different from that of the medium through which the light is traversing.
Unlike Rayleigh scattering, Mie scattering is not a physically independent phenomenon rather, it is a solution to Maxwell's equations for situations where the phase of the incident angle can change within the dimension of the scattering particles. Mie scattering is more commonly known as Mie solution, and it is named after Gustav Mie, a German physicist.
Mie scattering is also known as aerosol particle scattering, takes place in the atmosphere below 1,500 feet. In Mie scattering, the diameter of the spherical particles through which the light is scattered is approximately equal to the wavelength.

Applications

Mie scattering occurs in a variety of applications, including atmospheric science, cancer detection and treatment, metamaterials, and parasitology. Another application is the characterization of particles by optical scattering measurements.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Mie scattering measurement system

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