PROTEINDEX™ Dextrin Agarose 6 Fast Flow, 25 mL, 11-0218-025, Marvelgent biosciences (CAT#: STEM-C-3728-LGZ)

PROTEINDEX™ Dextrin Sepharose 6FF is a chromatographic medium for the purification of proteins fused to maltose-binding protein (mbp-tagged protein). Recombinant proteins with the MBP tag usually have higher expression levels and higher solubility. Proper folding of the attached protein was also shown to be facilitated by the MBP tag. Since MBP increases solubility, this tag is useful for recombinant proteins that tend to accumulate in an insoluble form (inclusion bodies).

Dextrin is the polymer product of amylose after amylase hydrolysis. Compared with amylose, which is easily degraded by amylase, dextrin is very stable. When dextrin was immobilized on the resin as a ligand substrate to purify the MBP tag, it had consistently strong affinity uptake even after repeated use.

Affinity purification using fast flow of Dextrin Sepharose 6 under physiological conditions and gentle elution using maltose preserves the activity of the target protein. These mild conditions can even purify intact protein complexes.

The base matrix of Dextrin Sepharose 6 Fast Flow is a firm, highly cross-linked 6% agarose. The cross-linking of bases has been optimized, which has high physical and chemical stability and high specific binding.

Our proprietary ProteIndex™ conjugation technology ensures stable immobilization of active ligands and minimal ligand leakage under a wide range of purification buffer conditions. This resin can be reused multiple times to purify the same target protein.

Size: 25 mL
Ligand: Dextrin
Binding capacity: >10 mg MBP tagged protein (80 kDa)/mL medium
Matrix: 6% highly cross-linked agarose supplied as 50% slurry
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar, 43 psi)
Recommended flow rate: 300 - 500 cm/hour
pH stability: 3-12
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol as preservative

High selectivity for high purity
> 10 mg MBP tagged protein (80 kDa)/mL medium
Ligand coupling is stable with low leakage; can be reused to purify the same protein

Chromatography Column, Fixed Volume, 5-7bar, 10mm ID, 800mm length, 7bar, GB-10-800, G-BIOSCIENCES (CAT#-STEM-C-3589-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 10mm ID, 900mm length, 7bar, GB-10-900, G-BIOSCIENCES (CAT#-STEM-C-3590-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 10mm ID, 1000mm length, 7bar, GB-10-1000, G-BIOSCIENCES (CAT#-STEM-C-3591-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 100mm length, 7bar, GB-15-100, G-BIOSCIENCES (CAT#-STEM-C-3592-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 200mm length, 7bar, GB-15-200, G-BIOSCIENCES (CAT#-STEM-C-3593-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 300mm length, 7bar, GB-15-300, G-BIOSCIENCES (CAT#-STEM-C-3594-LGZ)