Recombinant Human CD155 Protein, Seafrom (CAT#: STEM-CT-0134-ZJF)

The Recombinant Human CD155 Protein manufactured by Seafrom is suitable for immune cell activation and regulation.
Cluster of Differentiation 155 (CD155), also known as poliovirus receptor (PVR), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD155 is commonly referred to as the poliovirus receptor due to its involvement in the cellular entry of the poliovirus in primates. Its normal cellular function is to establish intercellular adhesive connections between epithelial cells. CD155/PVR was initially isolated based on its ability to mediate the attachment of the poliovirus to host cells.
The full-length (or CD155α isoform) is synthesized as a 417-amino acid (aa) precursor, which includes a 20 aa signal sequence, 323 aa extracellular region, 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. The extracellular region comprises an N-terminal V-type and two C2-type Ig-like domains. CD155 is a transmembrane protein with three extracellular immunoglobulin-like domains, namely D1-D3, where D1 is recognized by the virus. Low-resolution structures of CD155 in complex with poliovirus have been observed by electron microscopy, while high-resolution structures of the D1 and D2 domains of CD155 have been obtained using X-ray crystallography.
Storage Information:
The lyophilized (freeze-dried) form can be stored at 4°C for 24 months, while the reconstituted liquid can be stored at -20°C for 6-12 months, with a recommendation to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5
Protein Sequence: The DNA sequence encodes human CD155 (NCBI Reference Sequence: NP_006496.4), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human CD155 protein contains 323 amino acids, with a predicted theoretical molecular weight of 36.8 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: The binding activity was determined using functional ELISA. When human CD155/PVR (His tag) is immobilized at 2 μg/mL (100 μL/well), DNAM-1/CD226 (FC tag) generates 50% optimal binding activity in the range of 50-500 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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