The Recombinant SARS-CoV-2 Spike Protein-RBD (Fc Tag) manufactured by Seafrom is suitable for studying SARS-CoV-2 virus-host interactions. The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors that bind S1 include ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), and CEACAM (carcinoembryonic antigen-related cell adhesion molecule). The spike protein is a large type I transmembrane protein consisting of two subunits, S1 and S2. S1 primarily contains a receptor binding domain (RBD) responsible for recognizing the cell surface receptor, while S2 contains essential elements for membrane fusion. The S protein plays key roles in inducing neutralizing antibody and T-cell responses, contributing to protective immunity. The main functions of the Spike protein are summarized as follows: 1. Mediate receptor binding and membrane fusion. 2. Define the range of hosts and specificity of the virus. 3. Serve as the main component for binding with neutralizing antibodies. 4. Act as a key target for vaccine design. 5. Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), potentially leading to a higher mortality rate. Storage Information: 1. Lyophilized samples can be stored at 4°C for 24 months, and reconstituted liquid can be stored at -20°C for 6-12 months, while avoiding repeated freeze-thaw cycles. 2. Liquid samples can be stored at -20 to -80°C for 12 months, and after thawing, should be aliquoted and stored at -20°C for an additional 6-12 months, while avoiding repeated freeze-thaw cycles.
Specification
Synonyms: 2019-nCoV RBD Protein; 2019-nCoV S1 Protein; 2019-nCoV Spike RBD Protein Expression Host: CHO cells Protein Sequence: The DNA sequence encodes recombinant SARS-CoV-2 (2019-nCoV) RBD protein, expressed with an Fc tag at the C-terminus. Molecular Weight: Recombinant SARS-CoV-2 (2019-nCoV) RBD protein contains 470 amino acids, with a predicted theoretical molecular weight of 52.9 kD. Purity: ≥95%, confirmed by SDS-PAGE gel and size-exclusion high-performance liquid chromatography (SEC-HPLC) analysis. Endotoxin: ≤0.01 EU/μg (gel-clot method) Purification Method: Chromatographic purification Composition: Freeze-dried powder of sterile PBS (pH 7.4) containing 6% mannitol and 6% trehalose. Liquid of sterile PBS (pH 7.4) filtered through a 0.2 μM filter at a concentration of 0.2 μM.