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Study of action of peppermint oil on gastrointestinal smooth muscle by Patch clamp (CAT#: STEM-PET-0043-WXH)

Introduction

Peppermint oil is the volatile oil obtained from the overground parts of the flowering plant Me&ha x Piperita L. Traditionally, it has been used as a carminative agent, in the relief of flatulence and colic pain, and more recently in therapy of the irritable bowel syndrome. The major constituents of peppermint oil are menthol and menthone, although at least nine other constituents are known to be present in the natural oil. Peppermint oil has been shown to relax isolated gastrointestinal smooth muscle in both laboratory animals and humans.




Principle

The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal.

Applications

• Study of ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.
• Study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
• Study of ion channels.

Procedure

1. Fabrication of glass electrodes
2. Measuring glass electrode resistance and compensating offset potential
3. Glass electrode contact to cell membrane and obtain a GΩ seal
4. Acquire and analyse recordings using the appropriate software.

Materials

Patch clamp system
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