Study of BCR/ABL Rearrangements in Chronic Myeloid Leukaemia (CML) by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1208-WXH)

Introduction

Chronic myeloid leukaemia (CML) is a clonal myeloproliferative disorder of the haematopoietic stem cells. About 90% to 95% are characterised cytogenetically by the presence of the Philadelphia (Ph) chromosome, due to a translocation between chromosomes 9 and 22. This rearrangement results in the formation of a chimeric BCR/ ABL fusion gene on the derivative chromosome 22. The fusion gene is transcribed and spliced into a 8.5 kb chimeric messenger RNA with b2a2 and b3a2 configurations. The translation product is a 210 kD BCR/ABL protein that contributes to CML pathogenesis.