Study of cytoplasmic fragments from preimplantation human embryos by transmission electron microscopy technology (CAT#: STEM-MIT-0022-LJX)

Introduction

Embryonic fragmentation is a common phenomenon in the early embryonic development of mammals. It is a cytoplasmic structure enclosed by cell membrane and without nucleus that is discharged from a fertilized egg or early embryo. Some of these cytoplasmic structures contain organelles such as mitochondria, while others do not. Those fragments without organelles can be eliminated by ablation or reabsorption of the blastomere during the process of embryo division, and the location and distribution of fragments can be changed with the movement of the blastomere.
The formation of fragments is the result of apoptosis of embryonic cells. The more fragments of the embryo, the worse the growth potential of the embryo may be, which has become an important sign to judge the quality of the embryo.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cytoplasmic fragments from preimplantation human embryos

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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