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Observing ultrastructure of spermatozoa of tench Tinca tinca by transmission electron microscopy technology (CAT#: STEM-MIT-0030-LJX)

Introduction

The sperm structure of Tinca tinca L. was studied by transmission electron microscopy (TEM). Sperm with a total length of 26.1+/-3.8 microm have a typical primitive simple structure, called "hydrosperms", with no acrosomal head structure. It may be the smallest sperm ever described in a cyprinid fish. The head is mainly composed of dense and slightly grainy material, which looks fairly uniform except for occasional vacuoles. The midsection is separated from the flagella by cytoplasmic channels and is cylindrical/conical in shape. The vesicles are attached to the most basal region of the flagella, just below the plasma membrane of the flagella.

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Principle Applications Procedure Materials Notes

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Spermatozoa of tench Tinca tinca

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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