Study of Enzyme catalysis kinetics by Quench-Flow method (CAT#: STEM-AC-0032-WXH)

Introduction

Enzyme catalysis is a procedure to increase the rate of virtually all the chemical reactions within cells by the active site of a protein. Enzyme may be part of a multi-subunit complex. It may also transiently or permanently conjugate with a cofactor.

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Principle Applications Procedure Materials Notes Related Products

Principle

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Applications

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Procedure

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.

Materials

Quench flow

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