Study of primary breast carcer by Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0386-WXH)

Introduction

Breast cancer starts when cells in the breast begin to divide and grow in an unusual and uncontrolled way. Primary breast cancer is breast cancer that has not spread beyond the breast or the lymph nodes under the arm.

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Principle Applications Procedure Materials

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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