Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0370-WXH)

Introduction

Spectral karyotype (SKY) is a karyotype in which the homologous pairs of chromosomes are manipulated in such a way that they have distinctive colors. The SKY technique makes it easier for scientists to detect chromosomal abnormalities, as compared with a conventional karyotype.

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Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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