Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0370-WXH)

Introduction

Spectral karyotype (SKY) is a karyotype in which the homologous pairs of chromosomes are manipulated in such a way that they have distinctive colors. The SKY technique makes it easier for scientists to detect chromosomal abnormalities, as compared with a conventional karyotype.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

Other recommended products

Analysis of B-cell Neoplasias by Spectral Karyotyping (SKY) (CAT#: STEM-ST-0383-WXH)
Characterization of Chromosomal Rearrangements Using Spectral Karyotyping (CAT#: STEM-ST-0382-WXH)
Characterization of chromosomal abnormalities in prostate cancer cell lines by spectral karyotyping (CAT#: STEM-ST-0378-WXH)
Study on human hepatocellular carcinoma by spectral karyotyping (CAT#: STEM-ST-0374-WXH)
Karyotype analysis of human cytotrophoblasts (CAT#: STEM-ST-0385-WXH)
Multicolor Spectral Karyotyping of Human Chromosomes (CAT#: STEM-ST-0372-WXH)
Study of primary breast carcer by Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0386-WXH)
Spectral karyotyping of oocytes (CAT#: STEM-ST-0376-WXH)