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Study of Temperature Dependence of Elastic Properties of the Phospholipid Vesicles in Aqueous Suspension by Brillouin Scattering (CAT#: STEM-ST-0151-YJL)

Introduction

Phospholipid vesicles, also known as liposomes, are small spherical structures made up of a lipid bilayer. Phospholipid vesicles have a bilayer structure when dispersed in aqueous medium. Fatty acid chains of phospholipids concern formation of bilayers and result in different vesicle sizes under the same preparatory conditions and energy input. The lipid bilayer is composed of phospholipids, which have a hydrophilic (water-loving) head and a hydrophobic (water-fearing) tail. This structure allows liposomes to encapsulate both hydrophilic and hydrophobic molecules within their interior or between their bilayers. Small particle sizes have a greater advantage in colloidal stability. Liposomes have a wide range of applications in drug delivery, cosmetics, and research due to their ability to protect and transport molecules.




Principle

From a quantum point of view, Brillouin scattering is an interaction of light photons with acoustic or vibrational quanta (phonons), with magnetic spin waves (magnons), or with other low frequency quasiparticles interacting with light. The interaction consists of an inelastic scattering process in which a phonon or magnon is either created (Stokes process) or annihilated (anti-Stokes process). The energy of the scattered light is slightly changed, that is decreased for a Stokes process and increased for an anti-Stokes process. This shift, known as the Brillouin shift, is equal to the energy of the interacting phonon and magnon and thus Brillouin scattering can be used to measure phonon and magnon energies.

Applications

Brillouin scattering is used to determine acoustic velocities and elastic properties of a number of crystalline solids, glasses, and liquids.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Brillouin scattering measurement system (Brillouin spectrometer)