Study of Tn5 transposase by Magnetic tweezers (MT) (CAT#: STEM-MB-1271-WXH)

Introduction

The Tn5 transposase is an example of a class of proteins that move DNA sequences (transposons) via a process called transposition. DNA transposition is a widespread genetic mobility mechanism that has profoundly affected the genomes of nearly all organisms.
The movement of genetic material from place to place in the genome (transposition) mediated by transposase proteins (Tnp) has profoundly impacted the composition of genomes of many organisms. A key initial step in transposition is organization of Tnp and transposon DNA into an ordered, catalytically competent, and highly stable. nucleoprotein “synaptic complex”. Synaptic complex assembly is required for DNA cleavage to occur. Tnps in a synaptic complex cut the transposon from “donor” DNA and free it to be inserted elsewhere in the genome.