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Sugar Preference Test (CAT#: STEM-AE-0011-WXH)

Introduction

The sugar water preference experiment, also known as the double-bottle drinking choice experiment, is an animal behavioral experimental method for studying drug addiction (alcohol oral administration) and depressive behavior. Rodents naturally have a sweet taste preference, and when the mice exhibited depression-like behavior, their preference for sugar water was also reduced. Anhedonia is an important feature of mental illness and behavioral disorders. This is also what most depression patients describe as "the world is gray, and I can't feel what is happiness". Based on this principle, the sugar water preference test is used to evaluate the depression-like behavior of animals and the effect of antidepressant drugs.




Applications

Evaluate the depressive-like behavior of animals and the effect of antidepressant drugs.

Procedure

1. Raise the mice in a single cage and eat freely.
2. On the first day of the experiment, two bottles of drinking water were placed in each cage.
3. On the second day, switch to a bottle of 1% sucrose water and a bottle of drinking water.
4. On the third day, the positions of the two bottles are exchanged to adapt to the sugar water.
5. On the fourth day, the mice were fasted from food and water.
6. Place a weighed bottle of sugar water and a bottle of drinking water in each cage on the fifth day.
7. On the sixth day, weigh the amount of remaining water. The difference between the two times is the amount of drinking water and sucrose water. Finally, the sugar water preference is sucrose water/(drinking water + sucrose water)*100%.

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