Thermal unfolding (stability) studies of membrane proteins by Circular dichroism (CD) (CAT#: STEM-MB-0600-WXH)

Introduction

Often ligand binding produces little change in the peak magnitudes at room temperature, and thus cannot be used to assess whether or not the compound has bound. However, ligand binding often acts to stabilise the protein (and can discriminate between mutations that do and do not affect the binding interactions). This stabilisation can be assessed by thermal denaturation studies. Thermal denaturation studies have the advantage over chemical denaturation studies of leaving other environmental conditions such as ionic strength and pH intact