UniSil HILIC Series HILIC Phase Bulk Media, UniSil 10-120 Amide (CAT#: STEM-C-3153-ZJF)

The UniSil HILIC chromatography media offer exceptional separation performance across various hydrophilic interaction chromatography (HILIC) modes. These phases support mobile phases containing up to 60% aqueous content, making them particularly effective for separating highly polar or hydrophilic molecules that are challenging to retain using conventional reversed-phase chromatography.
Ideal for HPLC analysis, preparation, and purification of hydrophilic compounds, these media enhance the retention of strongly polar basic compounds without requiring toxic solvents like hexane or chloroform. Designed with advanced engineering, the HILIC media deliver high column efficiency, excellent selectivity, robust mechanical strength, outstanding chemical stability, and an extended service life. They serve as a strong complement to reversed-phase chromatography media, expanding the capabilities of chromatographic separation.

Matrix: Silica gel
Particle Size (μm): 3/5/8/10/20/30/50
Pore Size (Å): 120
Particle Size CV: ＜5%
Surface Area (m²/g): ~350
Pore Volume (cm³/g): ~0.95
Bulk Density (g/mL): ~0.50
Bonding Phase: Amide
Carbon Content (%): ~5
Max. Pressure (bar): ＜600
Working pH: 2-8
Operational Temperature (℃): 10-60
Storage: After use, clean with salt and seal in 100% ethanol or isopropanol; store at 4-25℃ in a cool, dry place.

Superior Separation in HILIC: UniSil series HILIC phases offer excellent separation performance for hydrophilic compounds using up to 60% aqueous mobile phase.
Ideal for Polar and Hydrophilic Compounds: Especially effective for separating strongly polar or hydrophilic molecules that are difficult to retain using traditional reversed-phase chromatography.
Enhanced Retention for Basic Compounds: Provides improved retention of strongly polar basic compounds without the need for toxic solvents like hexane or chloroform.
High Column Efficiency: UniSil HILIC media deliver high column efficiency, ensuring consistent and effective separations.
Excellent Selectivity and Stability: Features excellent selectivity, high mechanical strength, and chemical stability, contributing to longer service life.
Complementary to Reversed Phase Chromatography: The HILIC media offer strong complementarity with reversed phase chromatography, expanding the range of analytes that can be separated efficiently.

STEM-C-3151-ZJF
STEM-C-3152-ZJF
STEM-C-3154-ZJF
STEM-C-3155-ZJF