Analysis of Adenosine Deaminase (ADA) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1445-LGZ)

Substrate adenosine is hydrolyzed to hypoxanthine riboside under the action of adenosine deaminase (ADA), and hypoxanthine riboside is hydrolyzed under the catalysis of purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Hypoxanthine produces hydrogen peroxide under the action of xanthine oxidase, and hydrogen peroxide produces red substance under the action of peroxidase, 4-aminotipyrine and color source, with maximum absorption at wavelength 550 nm. The activity of adenosine deaminase can be obtained by detecting the rate of red substance production within unit time.

It is suitable for the determination of adenosine deaminase (ADA) activity in animal tissue and serum (plasma) samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue and serum (plasma) samples