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Analysis of Akt (Human, Mouse, Rat, Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0016-LGZ)

Introduction

As a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major concern in the medical community because of its important role in regulating a variety of cellular functions, including metabolism, growth, proliferation, survival, transcription, and protein synthesis.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Full length recombinant human Akt1, phosphorylated in vitro

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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