Differential Scanning Fluorimetry(DSF) to study protein stability (CAT#: STEM-MB-0234-WXH)

Introduction

Differential Scanning Fluorimetry (DSF) measures protein unfolding by monitory changes in fluorescence as a function of temperature. Conventional DSF uses a hydrophobic fluorescent dye that binds to proteins as they unfold. NanoDSF measures changes in intrinsic protein fluorescence as proteins unfold. With DSF you can quickly screen through many different proteins or formulations to find the most stable ones.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Protein stability assessment
• Protein structure studies
• Protein-ligand interaction studies
• High throughput screening of protein stabilizers and inhibitors
• Identification of biosimilar candidates
• High-throughput screening of enzyme stability
• Screening of membrane protein detergents
• Protein quality control
• Small molecule inhibition mechanism study

Procedure

1. Instrument Preparation
2. Sample Preparation
3. Reaction system configuration
4. Detection with instrument
5. Data analysis

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