Analysis of Aldehyde Dehydrogenase (ALDH) by Fluorescence Microplate Reader (CAT#: STEM-MB-1512-LGZ)

Introduction

Sensitivity: 0.06 μmol/L
Detection range: 0.06–3 μmol/L
Precision: inter-lot difference of 8.4%, intra-lot difference of 4%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

The substrate converts NAD+ into NADH under the action of acetaldehyde dehydrogenase, which converts the fluorescent probe into a fluorescent substance under the action of the enzyme. The fluorescent substance is excited at 535 nm and the emission wavelength is detected at 587 nm.

Applications

It is suitable for detecting the activity of acetaldehyde dehydrogenase (ALDH) in animal tissues, cells and serum (plasma).

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissues, cells and serum (plasma)

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