Analysis of ATG4B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1550-LGZ)

Introduction

Official Full Name: autophagy related 4B cysteine peptidase
Also known as: APG4B; AUTL1; HsAPG4B
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed within cells. Autophagy is considered essential for cellular homeostasis and cellular remodeling during cellular differentiation, metamorphosis, non-apoptotic cell death, and senescence. Reduced levels of autophagy have been described in some malignancies and a role for autophagy in the control of unregulated cell growth associated with cancer has been proposed. This gene encodes a member of the autophagy protein family. The encoded protein has also been designated as a member of the C-54 cysteine protease family. Alternative transcriptional splice variants encoding different isoforms have been characterized.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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