Analysis of Cell Proliferation by Flow Cytometry (CAT#: STEM-CBT-0058-WXH)

Introduction

Assays that measure cell proliferation in individual cells work by monitoring changes in intracellular markers and/or DNA. Flow cytometry is an ideal platform for measuring cell proliferation and enables us to study various unique cell types within a heterogeneous population.
Cell proliferation can be measured by flow cytometry using several different assays and markers.

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Principle Applications Procedure Materials Notes Related Products

Principle

These assays use different methods to target proliferation-related events such as:
1. Incorporation of thymidine analogs (BrdU) into replicating DNA
2. Generational tracking of inheritable permanent dyes (CFSE)
3. Expression of proliferation related antigens (Ki67, PCNA)

Applications

• Determine the influence of the drug on cell growth and proliferation.
• Support investigations into immunophenotyping, compound toxicity, drug effects on tumor cell growth, and T cell behavior under different conditions.
• Determine the influence of the drug on apoptpsis.

Procedure

1. Culture medium preparation
2. Mononuclear cell isolation
3. Cell staining
4. Incubate for desired length of time under growth conditions
5. Analyze using a flow cytometer

Materials

Ethynyl Deoxyuridine
BrdU (thymidine analogs)
CFSE (inheritable permanent dyes)
Expression of proliferation related antigens (Ki67, PCNA)

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