Immunophenoptyping by Flow Cytometry (CAT#: STEM-CBT-0063-WXH)

Introduction

Flow cytometry is most commonly used for immunophenotyping. Immunophenotyping is the analysis of heterogeneous populations of cells for the purpose of identifying the presence and proportions of the various populations of interest. Antibodies are used to identify cells by detecting specific antigens expressed by these cells, which are known as markers. These markers are usually functional membrane proteins involved in cell communication, adhesion, or metabolism. Immunophenotyping using flow cytometry has become the method of choice in identifying and sorting cells within complex populations, for example the analysis of immune cells in a blood sample. Applications of this technology are used both in basic research and clinical laboratories.