Analysis of DKK-3 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0132-LGZ)

Introduction

DKK (Dickkopf) is a group of secreted glycoproteins, including DKK-1-4 four members. DKKs have a signal sequence and two cysteine-rich conserved domains, namely CYS1 near the N-terminus and CYS2 near the C-terminus. Human DKK-1 is composed of 266 amino acids, and its relative molecular mass is about 29k. DKK-1 is an inhibitory molecule of Wnt pathway.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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