Analysis of FGF-7 (Human) by ELISA (CAT#: STEM-MB-0816-LGZ)

Introduction

Fibroblast growth factor 7 (FGF7) is a secreted protein found mainly in epithelial cells and belongs to the heparin-binding growth factor family. Members of the FGF family have broad mitotic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a highly efficient epithelial cell-specific growth factor, and its mitotic activity is mainly expressed in keratinocytes, but not in fibroblasts and endothelial cells. It may be a major paracrine effect of normal epithelial cell proliferation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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