Analysis of Grass Carp Reovirus (GCRV) by Real-Time PCR Method (CAT#: STEM-MB-3669-LGZ)

Introduction

Grass carp reovirus (GCRV) is a dsRNA virus; the genome of this virus contains 11 segmented dsRNAs. Infection of grass carp with GCRV leads to haemorrhagic disease, one of the most serious diseases in the grass carp aquaculture industry.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.