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Analysis of IL-34 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0344-LGZ)

Introduction

Interleukin 34 (IL-34) is a protein that is part of a group of cytokines known as interleukins. It was originally discovered in humans, and orthologs of interleukin-34 in chimpanzee, mouse, rat, and chicken were also discovered through large-scale screens of secreted proteins. The protein consists of 241 amino acids, has a mass of 39 kilodaltons, and forms homodimers. IL-34 increases the growth or survival of immune cells called monocytes, which it activates by binding to the colony-stimulating factor 1 receptor.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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