Analysis of LZTS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1915-LGZ)

Introduction

Official Full Name: leucine zipper tumor suppressor 1
Also known as: F37; FEZ1
This gene encodes a tumor suppressor protein that is ubiquitously expressed in normal tissues. In uveal melanomas, expression of this protein is silenced in rapidly metastasizing and metastatic tumor cells but has normal expression in slowly metastasizing or nonmetastasizing tumor cells. This protein may have a role in cell-cycle control by interacting with the Cdk1/cyclinB1 complex. This gene is located on chromosomal region 8p22. Loss of heterozygosity (LOH) in the 8p arm is a common characteristic of many types of cancer.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Vibrio Spp. by Real-Time PCR Method (CAT#: STEM-MB-3006-LGZ)
Analysis of Ampelopsis Radix by Real-Time PCR Method (CAT#: STEM-MB-3927-LGZ)
Analysis of Yersinia Enterocolitica by Real-Time PCR Method (CAT#: STEM-MB-3301-LGZ)
Analysis of Foot-and-Mouth Disease Virus (FMDV) - C by Real-Time PCR Method (CAT#: STEM-MB-3102-LGZ)
Analysis of LOC115947637 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1606-LGZ)
Analysis of OR52N5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2360-LGZ)
Analysis of SPTLC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1940-LGZ)
Analysis of CEP104 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1281-LGZ)