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Analysis of MCAM/CD146 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0393-LGZ)

Introduction

CD146, also known as melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a single chain membrane penetrating glycoprotein belonging to the immunoglobulin superfamily (Igsf) and homologous to many cell adhesion molecule (CAM). CD146 is active in a variety of cellular physiological processes, including development, signal transduction, cell migration, mesenchymal stem cell differentiation, angiogenesis, and immune response. Among them, the most studied one is involved in tumor angiogenesis and metastasis.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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