Under the catalysis of peroxidase, hydrogen peroxide can oxidize non-fluorescent probe into fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample.
Applications
It was used to detect the peroxidation activity of myeloperoxidase in animal tissue and serum (plasma) samples.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Determination of fluorescence intensity. 4. Make the mark curve and calculate the result.
Materials
• Sample Type: animal tissue and serum (plasma) samples