Analysis of Myeloperoxidase (MPO) Peroxidation Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1507-LGZ)

Introduction

Sensitivity: 0.001 U/L
Detection range: 0.001-1.26 U/L
Precision: inter-lot difference of 5.4%, intra-lot difference of 1%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Under the catalysis of peroxidase, hydrogen peroxide can oxidize non-fluorescent probe into fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample.

Applications

It was used to detect the peroxidation activity of myeloperoxidase in animal tissue and serum (plasma) samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue and serum (plasma) samples

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