Analysis of Non-esterified Free Fatty Acids (NEFA/FFA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1499-LGZ)

Introduction

Sensitivity: 0.15 mmol/L
Detection range: 0.15-1.5 mmol/L
Precision: inter-lot difference of 5.1%, intra-lot difference of 3.3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 690-730 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Under weakly acidic conditions, free fatty acids react with copper salts to produce copper soap, which has a characteristic absorption peak at 715 nm. The content of free fatty acids has a linear relationship with the degree of color development within a certain range, and the content of free fatty acids can be calculated.

Applications

It is used to detect the content of free fatty acids in animal tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissues

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