Analysis of NPY (Human) by ELISA (CAT#: STEM-MB-1099-LGZ)

Introduction

Neuropeptide Y (NPY) is a hormone that widely exists in the central and peripheral and maintains homeostasis. In the center, NPY has anti-anxiety and anti-epileptic functions, and has the functions of inhibiting reproduction, inhibiting muscle excitation, and inhibiting sympathetic excitation, resulting in a decrease in blood pressure, heart rate, and metabolism of the human body. It can also promote appetite, and thus become the target of dieting drugs . However, peripheral NPY has a positive stimulatory effect, and it enhances the stress response together with glucocorticoids and catecholamines. NPY can induce vasoconstriction and vascular smooth muscle proliferation in the periphery, leading to elevated blood lipids, glucose tolerance, and the release of adipocytokines.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration, Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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