Analysis of PIK3C2B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0572-LGZ)

Introduction

Official Full Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Also known as: C2-PI3K
The protein encoded by this gene belongs to the phosphoinosine 3-kinase (PI3K) family. PI3 kinase plays a role in signaling pathways of cell proliferation, oncogenic transformation, cell survival, cell migration and intracellular protein transport. The protein contains a lipid kinase catalytic domain and a C-terminal C2 domain, a feature of class II PI3 kinases. The C2 domain acts as a calcium-dependent phospholipid-binding motif that mediates protein translocation to membranes and possibly protein-protein interactions. The PI3 kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of the protein binds phospholipids but not Ca2+, suggesting that the enzyme may act in a calcium-independent manner.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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