Analysis of Pro-Collagen I α1 by ELISA (CAT#: STEM-MB-1520-LGZ)

Introduction

Type I collagen is the most abundant structural protein in connective tissues such as skin, bone and tendons. It is a synthetic procollagen molecule characterized by a 300 nm triple helical domain flanked by globular n-terminal and C-terminal propeptides. The triple helical domain contains the Gly-Xaa-Yaa triplet, where Xaa and Yaa are usually proline and hydroxyproline, respectively. Procollagen N and C protease activity removes prohelical peptides so that mature trihelices can self-assemble into collagen fibril, providing tensile strength to the tissue.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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