Analysis of RBP7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1400-LGZ)

Introduction

Official Full Name: retinol binding protein 7<br />Also known as: CRBP4; CRABP4; CRBPIV<br />The protein encoded by this gene is a member of the cellular retinol-binding protein (CRBP) family, whose members are required for vitamin A stability and metabolism. The encoded protein binds all-trans-retinol and is structurally similar to other CRBPs; however, it has a lower binding affinity for retinol than other CRBPs.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Madurella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3768-LGZ)
Analysis of KLHL20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1104-LGZ)
Analysis of Human Coronavirus (HCoV-OC43) by Real-Time PCR Method (CAT#: STEM-MB-3880-LGZ)
Analysis of Chlamydophila Pneumoniae by Real-Time PCR Method (CAT#: STEM-MB-2663-LGZ)
Analysis of Vibrio Cholerae by Real-Time PCR Method (CAT#: STEM-MB-3057-LGZ)
Analysis of DISC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0920-LGZ)
Analysis of Pseudomonas Spp. by Real-Time PCR Method (CAT#: STEM-MB-3159-LGZ)
Analysis of SULT1C3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1453-LGZ)