Analysis of TGF-β RII (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0572-LGZ)

Introduction

Transforming growth factor-β (TGF-β) belongs to a group of newly discovered TGF-β superfamily that regulates cell growth and differentiation. This cytokine transforms the phenotype of normal fibroblasts by altering their adherent growth characteristics in the presence of epidermal growth factor (EGF) to gain the ability to grow in AGAR without the density-dependent inhibition of growth.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Growth Factors, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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