Analysis of TNFRSF14 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1231-LGZ)

Introduction

Official Full Name: TNF receptor superfamily member 14
Also known as: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of A-GAMMA3'E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2202-LGZ)
Analysis of Helicobacter Spp. by Real-Time PCR Method (CAT#: STEM-MB-3295-LGZ)
Analysis of USH1C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2426-LGZ)
Analysis of Paenibacillus Larvae by Real-Time PCR Method (CAT#: STEM-MB-3528-LGZ)
Analysis of USP21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0732-LGZ)
Analysis of ATP1A1-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0133-LGZ)
Analysis of Equine Herpesvirus 3 (EHV-3) by Real-Time PCR Method (CAT#: STEM-MB-3181-LGZ)
Analysis of GAD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1494-LGZ)