Analysis of Viral Nervous Necrosis Virus (VNNV) by Real-Time PCR Method (CAT#: STEM-MB-3659-LGZ)

Introduction

Viral nervous necrosis (VNN) is caused by a betanodavirus, viral nervous necrosis virus (VNNV). VNNV is also referred to as viral encephalopathy and retinopathy (VER). VNNV can cause large economic losses in farmed fish populations due to high mortality rates in young and larval fish. VNNV affects the central nervous system of fish.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.