Cloned enzyme donor immunoassay To determine the quantity of analyte in a sample (CAT#: STEM-MB-0180-WXH)

Introduction

A cloned enzyme donor immunoassay (CEDIA) is a competitive homogenous enzyme immunoassay. This assay makes use of two component fragments of an enzyme which are each individually inactive. Under the right conditions in solution these fragments can spontaneously reassemble to form the active enzyme. For use in biochemical assays one of the enzyme fragments is attached to an analyte of interest.

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Principle Applications Procedure Materials Notes Related Products

Principle

A cloned enzyme donor immunoassay or CEDIA uses genetically engineered inactive fragments of beta‐ galactosidase. One fragment is bound to an analogue, and if the test antibody binds the labeled antigen, reformation of the enzyme fragments is inhibited. Conversely, if the labeled antigen does not interact with the antibody, as is the case with high concentration of the analyte in the sample, the enzyme fragments recombine and produce product from provided substrate.

Applications

Determine the quantity of analyte in a sample.

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