G-banding (CAT#: STEM-GT-0010-WXH)

Introduction

G-banding is a cytogenetics technique that helps determine the number and appearance of chromosomes by staining condensed metaphase chromosomes to produce a visible karyotype. When stained with Giemsa stain, the heterochromatic regions will present darker while the euchromatin sections will present light bands in G-banding. The chromosomes are stained in a distinctive pattern that allows the pairing of homologous chromosomes and is useful for verifying the genetic integrity of cells (primary cell and iPSCs) and for identifying structural abnormalities, through the photographic representation of the entire chromosome within the cell. It is well-known that long-term in vitro culture of cells and passaging introduces karyotypic/ chromosomal abnormalities in 20-50% of cells (e.g., insertions, deletions, duplications, translocations), and may lead to invasive cell populations that could affect cellular phenotype and integrity of results.