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Investigation of Polystyrene (PS) intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0742-WXH)

Introduction

Polystyrene (PS) is a synthetic polymer made from monomers of the aromatic hydrocarbon styrene. Polystyrene can be solid or foamed. Polystyrene Particles have been found to have numerous applications in scientific research, diagnostics, electronics, pharmaceuticals, and nanotechnology, such as purification and separation, and lateral flow detection. Functionalized polystyrene nanoparticles and polystyrene particles can be used in a variety of applications, such as drug delivery materials, bioimaging, markers, and immunoassays. With excellent stability, high binding capacity and ultra-high specificity (low background), polystyrene latex particles have been widely verified in academic and industrial scale immunoturbidimetric assays. Polystyrene particles provide a flexible platform for diagnostic and bio-separation applications.




Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.

Procedure

1. Sample preparation
2. Nanoparticle Tracking Analysis
3. NTA Data Processing, Display, and Interpretation

Materials

• Nanoparticle Tracking Analysis (NTA) instrument
• NanoSight instrument
• Nanoparticle Analyzer
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