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Karyotype Analysis (CAT#: STEM-CBT-0018-WXH)

Introduction

Karyotype analysis is performed to identify the cells with chromosomal and chromatid fragments, deletions and translocations, thereby assessing chromosome structure instability. Methods such as GTG staining and mFISH are widely used for karyotype abnormality analysis at different passages.

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Applications Procedure

Applications

• Detect balanced translocation
• Detect loss of heterozygosity
• Detect aneuploidy
• Detect low-frequency mosaicism

Procedure

1. Add colchicine to the cells in the logarithmic growth phase
2. Trypsinize and collect cells.
3. Hypotonicity
4. Pre-fixation
5. Fixation
6. Remove the supernatant
7. The slides were stained with Giemsa stain, rinsed with running water, mounted, observed under an oil microscope, and photographed for analysis.

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