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KLF4 Signaling Pathway Assay (CAT#: STEM-MB-0318-WXH)

Introduction

Kruppel-Like Factors (KLFs) are a family of transcription factors with zinc finger structure, which are involved in the regulation of several physiological activities and play a key role in apoptosis, differentiation, proliferation and embryonic development, and are closely related to eukaryotic gene transcriptional regulation.KLF4 is also known as gastrointestinal enriched Kruppel-like factor (GKLF) or epidermal zinc finger factor (EZF), Kruppel-like factor 4 (KLF4), as an important member of the KLFs family, shows high expression in primary hepatocellular carcinoma tissues, and KLF4 plays an important role in the biological function of primary hepatocellular carcinoma.




Principle

The amino terminus of KLF4 contains a highly variable transcriptional regulatory structural domain, containing a transcriptional activation structural domain between amino acid residues 91 and 117, rich in proline and serine, and a transcriptional repression structural domain between amino acid residues 181 and 388, rich in proline, indicating that KLF4 has two modes of activating and repressing gene transcription and plays different transcriptional regulatory function.

Applications

Study the regulatory mechanism of KLF4 signaling pathway in clinical medicine
Research on the influence of various medical viruses on KLF4 signaling pathway
Study the effects of carcinogenic drugs or carcinogenic therapies on KLF4 signaling pathway

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: IPS-1, ISGF3, MSK2, PI3K, TBK1, Vav, ATG13, ATG2, ATG12, ATG5, ATG7, ATG10, ATG3, ATG4, JNK, MYD88, Rac1, TLR4, ATG16, WIPi2, LC3I, PROLC3, ULK, FIP200, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, VPS15, VPS34, UYRAG, NFκB, RIG-1, TRAF3, GAS, IRF9, AMBRA1, p38, RIP1, TRAF5, IRAK1, ISRE, mTOR, PKR, TLR3

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