TCR Pathway Assay (CAT#: STEM-MB-0340-WXH)

Introduction

TCR (T cell receptor) is a T cell surface receptor, responsible for recognizing the specificity presented by major histocompatibility complex (MHC) on the surface of antigen presenting cell (APC) Sexual antigen peptide, forming TCR-pMHCcomplex (TCR-antigenic peptide-MHC complex, TCR-peptide-MHCcomplex).

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Principle Applications Procedure Materials Notes Related Products

Principle

There are three main pathways for T cell activation: phosphatidylimosito1 metabolic pathway, protein tyrosine kinase pathway, and T cell activation bypass pathway. In the latter two pathways, there are four main components that play a role in the initial stage of signal transduction. Species ZAP-70 (SykPTK), CD30 complex, Lck/Fyn (SrcPTK) and adaptor protein (1inker for activation of Tcell LAT).

Applications

To study the effects of drugs or therapies on TCR signaling pathways
To study the effect of each virus on TCR signaling pathway
To study the regulation mechanism of TCR signaling pathway in disease

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: PI3K, Lck, Zap-70, LAT, Grb2, SOS, PIP3, Digh1, HPK1, ADAP, Plcy1, SLP76, NCK, Vav, Wasp, Ras, CaM, CaMK4, Raf, MEKK1, PKC, PDK1, Akt, CBL2, Bcl10, Mail1, Carma1, TAK1, mTORC1, IKK, MKK7, MKK4, JNK2, p38MAPK, MEK1, MEK2, GADD45-α, JNK, Erk, CREB, NFAT, Fos, Rel, NF-kB, IKB, Jun

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