Lipocalin Signaling Pathway Assay (CAT#: STEM-MB-0371-WXH)

Introduction

Lipocalin-2 (LCN2), a member of the lipocalin superfamily, also known as 24p3, p25, MSFI neutrophil gelatinase-associated lipid transport protein NGAL, which can be expressed in a variety of cells. It can act as a transporter protein for lipophilic small molecules such as steroids, lipopolysaccharides, iron and fatty acids. It has the typical tertiary structure of the lipid transport protein family, i.e. a β-barrel structure formed by eight reversed parallel β-turns.
LCN2 is expressed in a variety of cells and is involved in the progression of multiple diseases and disorders. lCN2 plays an important role in the development, progression and metastasis of various cancers.

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Principle Applications Procedure Materials Notes Related Products

Principle

The most important LCN signal pathway cascades is the LCN2 signaling cascade. LCN2 is elevated in the inflammatory response and inflammatory state. Macrophages activate the inflammatory pathway of hepatocytes and induce a 17-fold increase in LCN2 levels. A range of pro-inflammatory factors promote LCN2 secretion, such as lipopolysaccharide, interleukin (IL)-1β, IL-17 and tumor necrosis factor alpha (TNF-α). In primary renal fibroblasts, lipopolysaccharide induces LCN2 expression via the activator protein 1 (AP-1) pathway, and CCAAT/enhancer binding protein δ (C/EBPδ) pathway induces longer-lasting expression of LCN2. Both pathways are dependent. IL-1β induces LCN2 mRNA expression via a nuclear factor κB (NF-κB) pathway.

Applications

Study the regulatory mechanism of LCN2 signaling pathway in clinical medicine
Research on the influence of various medical viruses on LCN2 signaling pathway
Study the effects of carcinogenic drugs or carcinogenic therapies on LCN2 signaling pathway

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: IPS-1, ATG5, Rac1, IRF9, AMBRA1, mTOR, ISGF3, ATG12, ULK, GAS, SH2, ISRE, MSK2, ATG2, MYD88, FIP200, p38MAPK, IRAK1, PI3K, ATG13, JNK, PKR, Histone-H3, TRAF6, TBK1, TRAF3, ATG4, TLR3, TRAF5, , Vav, RIG-1, ATG3, WIPi2, RIP1, , VPS15, NFκB, ATG10, ATG16, IRF5, MEK3, , VPS34, UYRAG, ATG7, TLR4, p38

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